In vivo proton observed carbon edited (POCE) (13) C magnetic resonance spectroscopy of the rat brain using a volumetric transmitter and receive-only surface coil on the proton channel.
|Title||In vivo proton observed carbon edited (POCE) (13) C magnetic resonance spectroscopy of the rat brain using a volumetric transmitter and receive-only surface coil on the proton channel.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Kumaragamage C, Madularu D, Mathieu AP, De Feyter H, Maria Rajah N, Near J|
|Journal||Magn Reson Med|
|Date Published||2017 May 12|
PURPOSE: In vivo carbon-13 ((13) C) MR spectroscopy (MRS) is capable of measuring energy metabolism and neuroenergetics, noninvasively in the brain. Indirect ((1) H-[(13) C]) MRS provides sensitivity benefits compared with direct (13) C methods, and normally includes a (1) H surface coil for both localization and signal reception. The aim was to develop a coil platform with homogenous B1+ and use short conventional pulses for short echo time proton observed carbon edited (POCE) MRS.METHODS: A (1) H-[(13) C] MRS coil platform was designed with a volumetric resonator for (1) H transmit, and surface coils for (1) H reception and (13) C transmission. The Rx-only (1) H surface coil nullifies the requirement for a T/R switch before the (1) H preamplifier; the highpass filter and preamplifier can be placed proximal to the coil, thus minimizing sensitivity losses inherent with POCE-MRS systems described in the literature. The coil platform was evaluated with a PRESS-POCE sequence (TE = 12.6 ms) on a rat model.RESULTS: The coil provided excellent localization, uniform spin nutation, and sensitivity. (13) C labeling of Glu-H4 and Glx-H3 peaks, and the Glx-H2 peaks were observed approximately 13 and 21 min following the infusion of 1-(13) C glucose, respectively.CONCLUSION: A convenient and sensitive platform to study energy metabolism and neurotransmitter cycling is presented. Magn Reson Med, 2017. © 2017 International Society for Magnetic Resonance in Medicine.
|Alternate Journal||Magn Reson Med|