Visualization of CXCR7 in the mouse hippocampus
Description and scientific context:
In this picture, a homozygote fluorescent receptor knock in mouse permitted us to see CXCR7 at regional levels in the brain. The seven-transmembrane receptor CXCR7 is a member of the chemokine G-protein coupled receptor family. Among its various functions in key processes in normal physiology, it regulates diseases including cancer, atherosclerosis, autoimmunity, and HIV infection, making these proteins promising drug targets. In the brain, key functions are neurogenesis, neuromodulation and brain inflammation. As a result of immunohistochemistry experiments examining the cell subtype of CXCR7 expressing cells we found precise expression in the endothelial cells along the blood vessels in the cerebral Cortex (CTX) and the cell bodies of neurons in the Hippocampus/Dentate Gyrus (DG). Here, you can see CXCR7 in two cell populations, endothelial cells along brain vasculature and neuronal cells in hippocampal CA2 and DG, exhibiting the high-level of CXCR7 expression in those particular b
Specimen and labels:
Cxcr7Venus/Venus mouse brains were coronally sliced. The tissue was then stained with GFP as a primary antibody combined with secondary antibody alexa fluor 594 (red) and DAPI to visualize nuclei.
Image acquired on Olympus VS12 slide scanner at 10x magnification.