Description and scientific context:
In this picture we see intricate projections labelled with green fluorescent protein around the large serotonergic neurons of the mouse dorsal raphe. In this experiment, we stereotaxically injected a Cre-recombinase dependent adeno-associated virus encoding EGFP into the medial habenula. A subset of medial habenula cells were fluorescently labelled, thus allowing us to trace both previously observed neuronal projections from this region to the interpeduncular nucleus, and newly detected projections to the dorsal raphe..
Specimen and labels:
EGFP labelled neuronal projections, Cy5 labelled serotonergic neurons (stained for tryptophan hydroxylase), and DAPI stained nuclei in a fixed brain slice from mouse dorsal raphe
Epifluorescence microscopy on an Olympus IX73, 10x magnification