White and Gray Matter Abnormalities After Cranial Radiation in Children and Mice.

TitleWhite and Gray Matter Abnormalities After Cranial Radiation in Children and Mice.
Publication TypeJournal Article
Year of Publication2015
AuthorsNieman BJ, A de Guzman E, Gazdzinski LM, Lerch JP, M Chakravarty M, Pipitone J, Strother D, Fryer C, Bouffet E, Laughlin S, Laperriere N, Riggs L, Skocic J, Mabbott DJ
JournalInt J Radiat Oncol Biol Phys
Volume93
Issue4
Pagination882-91
Date Published2015 Nov 15
ISSN1879-355X
KeywordsAnimals, Case-Control Studies, Cerebral Cortex, Child, Cranial Irradiation, Gray Matter, Hippocampus, Humans, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL, Models, Animal, Radiation Dosage, Radiation Injuries, Radiation Injuries, Experimental, Retrospective Studies, Time Factors, White Matter
Abstract

PURPOSE: Pediatric patients treated with cranial radiation are at high risk of developing lasting cognitive impairments. We sought to identify anatomical changes in both gray matter (GM) and white matter (WM) in radiation-treated patients and in mice, in which the effect of radiation can be isolated from other factors, the time course of anatomical change can be established, and the effect of treatment age can be more fully characterized. Anatomical results were compared between species.METHODS AND MATERIALS: Patients were imaged with T1-weighted magnetic resonance imaging (MRI) after radiation treatment. Nineteen radiation-treated patients were divided into groups of 7 years of age and younger (7-) and 8 years and older (8+) and were compared to 41 controls. C57BL6 mice were treated with radiation (n=52) or sham treated (n=52) between postnatal days 16 and 36 and then assessed with in vivo and/or ex vivo MRI. In both cases, measurements of WM and GM volume, cortical thickness, area and volume, and hippocampal volume were compared between groups.RESULTS: WM volume was significantly decreased following treatment in 7- and 8+ treatment groups. GM volume was unchanged overall, but cortical thickness was slightly increased in the 7- group. Results in mice mostly mirrored these changes and provided a time course of change, showing early volume loss and normal growth. Hippocampal volume showed a decreasing trend with age in patients, an effect not observed in the mouse hippocampus but present in the olfactory bulb.CONCLUSIONS: Changes in mice treated with cranial radiation are similar to those in humans, including significant WM and GM alterations. Because mice did not receive any other treatment, the similarity across species supports the expectation that radiation is causative and suggests mice provide a representative model for studying impaired brain development after cranial radiation and testing novel treatments.

DOI10.1016/j.ijrobp.2015.07.2293
Alternate JournalInt. J. Radiat. Oncol. Biol. Phys.
PubMed ID26530758
Grant List / / Canadian Institutes of Health Research / Canada

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