Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum.
Title | Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Cutando L, Puighermanal E, Castell L, Tarot P, Bertaso F, Bonnavion P, d'Exaerde Ade Kerchov, Isingrini E, Galante M, Dallérac G, Pascoli V, Lüscher C, Giros B, Valjent E |
Journal | Addict Biol |
Pagination | e12995 |
Date Published | 2020 Dec 26 |
ISSN | 1369-1600 |
Abstract | Prescription stimulants, such as d-amphetamine or methylphenidate are used to treat suffering from attention-deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) and cause phosphorylation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluA1 in the striatum. Whether other brain regions are also affected remains elusive. Here, we demonstrate that d-amphetamine and methylphenidate increase phosphorylation at Ser845 (pS845-GluA1) in the membrane fraction of mouse cerebellum homogenate. We identify Bergmann glial cells as the source of pS845-GluA1 and demonstrate a requirement for intact NE release. Consequently, d-amphetamine-induced pS845-GluA1 was prevented by β1-adenoreceptor antagonist, whereas the blockade of DA D1 receptor had no effect. Together, these results indicate that NE regulates GluA1 phosphorylation in Bergmann glial cells in response to prescription stimulants. |
DOI | 10.1111/adb.12995 |
Alternate Journal | Addict Biol |
PubMed ID | 33368923 |
Grant List | 201803PJT / / CIHR / / / Brain and Behavior Research Foundation / DOPAFEAR / / Agence nationale de la recherche / EPITRACES / / Agence nationale de la recherche / #113-2016 / / La Marató de TV3 Fundació / DEQ20160334919 / / Fondation pour la Recherche Médicale / / / Inserm / |