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Accueil > Minimum echo time PRESS-based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses.

Minimum echo time PRESS-based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses.

TitleMinimum echo time PRESS-based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses.
Publication TypeJournal Article
Year of Publication2018
AuthorsKumaragamage C [1], Madularu D [2], Mathieu AP [3], Lupinsky D [4], de Graaf RA [5], Near J [6]
JournalMagn Reson Med
Date Published2018 Feb 09
ISSN1522-2594
Abstract

PURPOSE: Indirect C MRS by proton-observed carbon editing (POCE) is a powerful method to study brain metabolism. The sensitivity of POCE-MRS can be enhanced through the use of short TEs, which primarily minimizes homonuclear J-evolution related losses; previous POCE-MRS implementations use longer than optimal echo times due to sequence limitations, or short TE image selected in vivo spectroscopy-based multi-shot acquisitions for 3D localization. To that end, this paper presents a novel single-shot point resolved spectroscopy (PRESS)-localized POCE-MRS sequence that involves the application of simultaneous editing and localization pulses (SEAL)-PRESS, allowing the TE to be reduced to a theoretically optimal value of ∼ 1/J .METHODS: The optimized SEAL-PRESS sequence was first evaluated in simulation and in phantom; next, the sequence was validated with dynamic in vivo POCE-MRS performed in a rat preparation during a 1,6- C -Glc infusion, and on a microwave fixed rat brain following a 2-hour [1,6- C ]-Glc infusion. POCE spectra from the SEAL-PRESS sequence were compared against a previously described 12.6-ms PRESS-POCE sequence utilizing a classical carbon editing scheme.RESULTS: The SEAL-PRESS sequence provides > 95% editing efficiency, optimal sensitivity, and localization for POCE MRS with an overall sequence TE of 8.1 ms. Signal amplitude of C-labeled metabolites Glu-H4, Gln-H4, Glx-H3, Glc-H6 +Glx-H2, and Asp-H2 were shown to be improved by >17% relative to a 12.6-ms PRESS-POCE sequence in vivo.CONCLUSION: We report for the first time, a single-shot PRESS-localized and edited 8.1-ms TE POCE-MRS sequence with optimal sensitivity, editing efficiency, and localization.

DOI10.1002/mrm.27119 [7]
Alternate JournalMagn Reson Med
PubMed ID29427395 [8]

Source URL: https://douglas.research.mcgill.ca/fr/minimum-echo-time-press-based-proton-observed-carbon-edited-poce-mrs-rat-brain-using-simultaneous

Links
[1] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=4591
[2] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=7265
[3] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=8661
[4] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=7289
[5] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=9700
[6] https://douglas.research.mcgill.ca/fr/publications?f%5Bauthor%5D=3416
[7] http://dx.doi.org/10.1002/mrm.27119
[8] https://www.ncbi.nlm.nih.gov/pubmed/29427395?dopt=Abstract