The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor.
|Title||The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor.|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Weaver ICG, Hellstrom IC, Brown SE, Andrews SD, Dymov S, Diorio J, Zhang T-Y, Szyf M, Meaney MJ|
|Journal||Philos Trans R Soc Lond B Biol Sci|
|Date Published||2014 Sep 26|
|Keywords||Animals, Blotting, Western, Chromatin Immunoprecipitation, DNA Primers, DNA-Binding Proteins, Early Growth Response Protein 1, Gene Knockdown Techniques, HEK293 Cells, Hippocampus, Humans, In Situ Hybridization, Maternal Behavior, Mutagenesis, Site-Directed, Rats, Rats, Long-Evans, Real-Time Polymerase Chain Reaction, Receptors, Glucocorticoid, Signal Transduction, Transcriptional Activation|
Variations in maternal care in the rat influence the epigenetic state and transcriptional activity of glucocorticoid receptor (GR) gene in the hippocampus. The mechanisms underlying this maternal effect remained to be defined, including the nature of the relevant maternally regulated intracellular signalling pathways. We show here that increased maternal licking/grooming (LG), which stably enhances hippocampal GR expression, paradoxically increases hippocampal expression of the methyl-CpG binding domain protein-2 (MBD2) and MBD2 binding to the exon 17 GR promoter. Knockdown experiments of MBD2 in hippocampal primary cell culture show that MBD2 is required for activation of exon 17 GR promoter. Ectopic co-expression of nerve growth factor-inducible protein A (NGFI-A) with MBD2 in HEK 293 cells with site-directed mutagenesis of the NGFI-A response element within the methylated exon 17 GR promoter supports the hypothesis that MBD2 collaborates with NGFI-A in binding and activation of this promoter. These data suggest a possible mechanism linking signalling pathways, which are activated by behavioural stimuli and activation of target genes.
|Alternate Journal||Philos. Trans. R. Soc. Lond., B, Biol. Sci.|
|PubMed Central ID||PMC4142034|
|Grant List||/ / Canadian Institutes of Health Research / Canada|