Global and Site-Specific Changes in 5-Methylcytosine and 5-Hydroxymethylcytosine after Extended Post-mortem Interval.

TitleGlobal and Site-Specific Changes in 5-Methylcytosine and 5-Hydroxymethylcytosine after Extended Post-mortem Interval.
Publication TypeJournal Article
Year of Publication2016
AuthorsGross JA, Nagy C, Lin L, Bonneil É, Maheu M, Thibault P, Mechawar N, Jin P, Turecki G
JournalFront Genet
Volume7
Pagination120
Date Published2016
Abstract

There has been a growing interest in the study of epigenetic mechanisms to elucidate the molecular bases of human brain-related diseases and disorders. Frequently, researchers utilize post-mortem tissue with the assumption that post-mortem tissue decay has little or no effect on epigenetic marks. Although previous studies show no effect of post-mortem interval on certain epigenetic marks, no such research has been performed on cytosine modifications. In this study, we use DNA from the brains of adult Sprague Dawley rats subjected to post-mortem intervals at room temperature, ranging from 0 to 96 h, to assess the stability of cytosine modifications, namely 5-methycytosine and 5-hydroxymethylcytosine. Our results indicate that neither global nor site-specific levels of 5-methycytosine and 5-hydroxymethylcytosine are affected by the post-mortem intervals we studied. As such, the use of post-mortem tissue to study cytosine modifications in the context of neurological or neuropsychiatric disorders is appropriate.

DOI10.3389/fgene.2016.00120
Alternate JournalFront Genet
PubMed ID27446202
PubMed Central IDPMC4917525
Grant ListR01 DA033684 / DA / NIDA NIH HHS / United States