Fluorescence-based cell-specific detection for laser-capture microdissection in human brain.
| Title | Fluorescence-based cell-specific detection for laser-capture microdissection in human brain. |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Rocco BR, Oh H, Shukla R, Mechawar N, Sibille E |
| Journal | Sci Rep |
| Volume | 7 |
| Issue | 1 |
| Pagination | 14213 |
| Date Published | 2017 Oct 27 |
| ISSN | 2045-2322 |
| Abstract | Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis. |
| DOI | 10.1038/s41598-017-14484-9 |
| Alternate Journal | Sci Rep |
| PubMed ID | 29079825 |
| PubMed Central ID | PMC5660154 |
| Grant List | R01 MH077159 / MH / NIMH NIH HHS / United States |