Fluorescence-based cell-specific detection for laser-capture microdissection in human brain.
|Title||Fluorescence-based cell-specific detection for laser-capture microdissection in human brain.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Rocco BR, Oh H, Shukla R, Mechawar N, Sibille E|
|Date Published||2017 Oct 27|
Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.
|Alternate Journal||Sci Rep|
|PubMed Central ID||PMC5660154|
|Grant List||R01 MH077159 / MH / NIMH NIH HHS / United States|