DNA methylation signatures triggered by prenatal maternal stress exposure to a natural disaster: Project Ice Storm.

TitleDNA methylation signatures triggered by prenatal maternal stress exposure to a natural disaster: Project Ice Storm.
Publication TypeJournal Article
Year of Publication2014
AuthorsCao-Lei L, Massart R, Suderman MJ, Machnes, iv Z, Elgbeili G, Laplante DP, Szyf M, King S
JournalPLoS One
Volume9
Issue9
Paginatione107653
Date Published2014
ISSN1932-6203
KeywordsChild, Disasters, DNA Methylation, Epigenesis, Genetic, Female, Humans, Male, Maternal Exposure, Pregnancy, Prenatal Exposure Delayed Effects, Stress, Psychological
Abstract

BACKGROUND: Prenatal maternal stress (PNMS) predicts a wide variety of behavioral and physical outcomes in the offspring. Although epigenetic processes may be responsible for PNMS effects, human research is hampered by the lack of experimental methods that parallel controlled animal studies. Disasters, however, provide natural experiments that can provide models of prenatal stress.METHODS: Five months after the 1998 Quebec ice storm we recruited women who had been pregnant during the disaster and assessed their degrees of objective hardship and subjective distress. Thirteen years later, we investigated DNA methylation profiling in T cells obtained from 36 of the children, and compared selected results with those from saliva samples obtained from the same children at age 8.RESULTS: Prenatal maternal objective hardship was correlated with DNA methylation levels in 1675 CGs affiliated with 957 genes predominantly related to immune function; maternal subjective distress was uncorrelated. DNA methylation changes in SCG5 and LTA, both highly correlated with maternal objective stress, were comparable in T cells, peripheral blood mononuclear cells (PBMCs) and saliva cells.CONCLUSIONS: These data provide first evidence in humans supporting the conclusion that PNMS results in a lasting, broad, and functionally organized DNA methylation signature in several tissues in offspring. By using a natural disaster model, we can infer that the epigenetic effects found in Project Ice Storm are due to objective levels of hardship experienced by the pregnant woman rather than to her level of sustained distress.

DOI10.1371/journal.pone.0107653
Alternate JournalPLoS ONE
PubMed ID25238154
PubMed Central IDPMC4169571
Grant ListMOP-1150067 / / Canadian Institutes of Health Research / Canada

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