Fluorescence-based cell-specific detection for laser-capture microdissection in human brain.

TitleFluorescence-based cell-specific detection for laser-capture microdissection in human brain.
Publication TypeJournal Article
Year of Publication2017
AuthorsRocco BR, Oh H, Shukla R, Mechawar N, Sibille E
JournalSci Rep
Volume7
Issue1
Pagination14213
Date Published2017 Oct 27
ISSN2045-2322
Abstract

Cell-specific molecular investigations of the human brain are essential for understanding the neurobiology of diseases, but are hindered by postmortem conditions and technical challenges. To address these issues we developed a multi-label fluorescence in situ hybridization protocol and a novel optical filter device to identify cell types and control for tissue autofluorescence. We show that these methods can be used with laser-capture microdissection for human brain tissue cell-specific molecular analysis.

DOI10.1038/s41598-017-14484-9
Alternate JournalSci Rep
PubMed ID29079825
PubMed Central IDPMC5660154
Grant ListR01 MH077159 / MH / NIMH NIH HHS / United States