Description and scientific context:
This image shows metabotropic glutamate receptor subtype 5 (mGluR5) and cells expressing dopamine D1 receptors in the striatum. mGluR5 regulates synaptic activity and plasticity, and is important for responses to drugs like cocaine and amphetamine. Our goal was to better understand the receptor’s functional mechanisms through its localization on specific cell types. The image shows mGluR5 along dendrites of D1Rexpressing neurons, where it might modulate inputs to these cells. There are also many instances of mGluR5 not associated with D1R+ cells, suggesting expression on other neurons or cell types and underlining the diversity of pathways this receptor may act through.
Specimen and labels:
Fixed mouse brain slice with tdTomato-labelled D1R-expressing neurons (red), mGluR5 receptors labelled using immunofluorescence with a mouse anti-mGluR5 antibody and fluorochrome-coupled secondary antibody (green), and Hoechst DNA stain marking nuclei (blue).
Confocal microscopy on an Olympus FV1200, 60x magnification with 3x optical zoom