Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region.
|Title||Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region.|
|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||King L, Robins S, Chen G, Turecki G, Zelkowitz P|
|Journal||Methods Mol Biol|
Oxytocin and its paralogue, vasopressin, are widely studied biomarkers in relation to pregnancy and birth, maternal and social behavior, and mental health. Epigenetics is a biological mechanism that mediates the link between environmental influences and behavioral patterns. In a candidate gene approach, we describe here a DNA methylation assay of two regions within the oxytocin system, using human buccal cells and next-generation sequencing. Two nanograms of DNA were sufficient to assess the DNA methylation status of 28 CpG sites (22 corresponding to the oxytocin receptor and 6 corresponding to the vasopressin/oxytocin intergenic region). This method proved to be non-invasive for the participants, and reproducible; its validity remains to be confirmed alongside other biomarkers of gene function.
|Alternate Journal||Methods Mol Biol|
|Grant List||MOP-123354 / / CIHR / Canada|