1st prize: The Roots of Thought
Description and scientific context:
In this picture, we see dopaminergic neurons that have been differentiated from induced pluripotent stem cells stained for linage-specific markers. The neurons can clearly be seen forming connections with each other, almost like the roots of trees. I analyzed the difference in how these markers are expressed in cells derived from patients and healthy controls to help us understand how deficits in dopamine production might occur during development. We believe that the differentiation of dopaminergic neurons can be affected by a variety of environmental and genetic factors during development, and that these can contribute to a variety of disease later in life.
Specimen and labels:
Tyrosine Hydroxylase (in green) and TUJ1 (in red) labelled dopaminergic neurons derived from induced pluripotent stem cells. Nuclei are stained with DAPI (blue).
Confocal microscopy on an Olympus FV100, FOV: 100x magnification