Salah El Mestikawy

Salah El-Mestikawy
 

Office: 
Perry E-3213

6875 Boulevard Lasalle
Verdun, QC
H4H 1R3


(514) 761-6131 x6151

Fax: 
(514) 762-3034


Research area: 
Basic Neuroscience / Animal Research

Research Theme: 
Stress, mood and impulsivity disorders

Lab images - click to enlarge: 
El-Mestikawy lab Feb 2015
Salah El-Mestikawy image 1
Salah El-Mestikawy image 2
El-Mestikawy lab May 2015
El-Mestikawy lab - mouse
VGlut3  3D

PhD
Professor, Department of Psychiatry, McGill University



Glutamatergic systems in normal and pathological brains


Our team investigates glutamatergic systems in normal and pathological brains of rodent and humans.

• Glutamate is the major excitatory transmitter in the brain. Before its exocytotic release glutamate is uploaded inside synaptic vesicles by carriers named VGLUT1-3. These transporters are key anatomical and functional markers of glutamatergic transmission 1. 
• VGLUTs are distributed in distinct neuronal populations. VGLUT1 and VGLUT2 are synthesized by glutamatergic neurons from cortical and subcortical areas 2. Surprisingly, VGLUT3 is expressed by cholinergic interneurons from the striatum, subpopulation of GABAergic interneurons from the cortex and the hippocampus and in serotoninergic neurons 3. This distribution suggested the existence of glutamate cotransmission with acetylcholine (Ach) or 5-HT or even GABA and brought up numerous questions on the physiological and pathophysiological implication of VGLUT3 1. 
• Our major objective is to understand the functional implication of glutamatergic systems in the normal and pathological brain.
• Our research is focused on:
1-    Normal and pathological aging
2-    Reward system and addiction
3-    Mood disorders (anxiety, trama)
• We work on rodent (biochemistry, molecular biology, anatomy, electrophysiology, opto and pharmacogenetic, behavior) as well on human (postmortem anatomy, genetic).
• We hope that this program will help to increase our understanding of the mammalian brain and also open new avenue for the treatment of brain disorders.

References
1.    El Mestikawy, S., Wallen-Mackenzie, A., Fortin, G.M., Descarries, L. & Trudeau, L.E. From glutamate co-release to vesicular synergy: vesicular glutamate transporters. Nat Rev Neurosci 12, 204-216 (2011).
2.    Herzog, E., et al. The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 21, RC181 (2001).
3.    Gras, C., et al. A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 22, 5442-5451 (2002).
4.    Gras, C., et al. The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone. Nat Neurosci 11, 292-300 (2008).


HERZOG E., BELLENCHI G. C., GRAS C., BERNARD V., RAVASSARD P., BEDET C., GASNIER B., GIROS B. AND EL MESTIKAWY S. The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons. J. Neurosci. 21 RC181: 1-6 (2001).

GRAS C., HERZOG E., BELLENCHI G. C., BERNARD V., RAVASSARD P., POHL M., GASNIER B., GIROS B. AND EL MESTIKAWY S. A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons. J. Neurosci. 22 : 5442-5451 (2002).

KASHANI A., BETANCUR B., GIROS B., HIRSCH E. AND EL MESTIKAWY S. Altered expression of vesicular glutamate transporters VGLUT1 and VGLUT2 in Parkinson disease. Neurobiology of aging, 28: 568-78 (2007).

KASHANI A., LEPICARD E., POIREL O., VIDEAU C., DAVID J.P., FALLET-BIANCO C., SIMON A., DELACOURTE A., GIROS B., EPELBAUM J., BETANCUR C. and EL MESTIKAWY S. Loss of VGLUT1 and VGLUT2 in the prefrontal cortex is correlated with cognitive decline in Alzheimer disease. Neurobiology of Aging, 29(11):1619-30. (2008).

GRAS C., AMILHON B., LEPICARD E., KEMEL M.L., HERBIN M., DUMAS S., VINATIER J., TZAVARA E., NOMIKOS G., POIREL O., GASNIER B., GIROS B. AND EL MESTIKAWY S. The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone. Nature Neuroscience 11(3): 292-300 (2008). (F1000)

AMILHON B., LEPICARD E., RENOIR T., MONGEAU R., POPA D., POIREL O., MIOT S., GRAS C., GARDIER A. M., GALLEGO J., HAMON M., LANFUMEY L., GASNIER B., GIROS B. AND EL MESTIKAWY S. VGLUT3 contribution to the regulation of serotonergic transmission and anxiety. J. Neuroscience, 30 (6): 2198-2210 (2010).

EL MESTIKAWY S., WALLÉN-MACKENZIE A., FORTIN G., DESCARRIES L. AND LOUIS-ERIC TRUDEAU L-E. From glutamate co-release to vesicular synergy: vesicular glutamate transporters. Nature Reviews Neurosci, 12 (4): 204-216 (2011).

VIGNEAULT E., POIREL O., RIAD M., DUMAS S., TURECKI G., FASANO C., MECHAWAR N.* and EL MESTIKAWY S.* Distribution of vesicular glutamate transporters in the human brain. *Equal contribution, corresponding authors. Frontier in Neuroanatomy 2015 Mar 5; 9-23. doi: 10.3389/fnana.2015.00023. eCollection 2015. PMID: 25798091 (fév 2015).

SAKAE D.Y.*, MARTI F.*, LECCA S.*, VORSPAN F., MARTIN-GARCIA E., MOREL L.J., HENRION A., GUTIERREZ-CUESTA J., BESNARD A., HECK N., HERZOG E., BOLTE S., PRADO V.F., PRADO M.A., BELLIVIER F., EAP C.B., CRETTOL S., VANHOUTTE P., CABOCHE P., GRATTON A., MOQUIN L., GIROS B., MALDONADO R., DAUMAS S., MAMELI M., JAMAIN S.* and EL MESTIKAWY S*. The absence of the vesicular glutamate transporter VGLUT3 predisposes to cocaine abuse by increasing dopamine and glutamate signaling in the nucleus accumbens. Mol Psy. 2015 Aug 4. doi: 10.1038/mp.2015.104. (july 2015). * Equal contribution.

 


•    Ph. D. in Neurosciences, Université P&M Curie, Paris, France, 1982
•    Habilitation à diriger des recherches, Université P&M Curie, Paris, France, 1991
•    Permanent position at the Centre National de la Recherche Scientifique (CNRS), France, 1983
•    Promoted Chargé de recherche 1ère classe, 1987
•    Promoted Directeur de recherche 2ième classe, 1995
•    Professor, McGill University (Canada Research Chair), 2008
•    Promoted Directeur de recherche 1ère classe, 2012
•    1978-1979, Laboratoire de Biochimie Cellulaire, Collège de France, Paris, Master of Science
•    1979-1985 INSERM U114, Collège de France, Paris, PhD
•    1985-1990 INSERM U288, Faculté de Médecine Pitié-Salpêtrière, Paris,
•    1990-1992 Dept of Cell Biology, Duke University, Durham, NC, USA, Postdoctoral training
•    1992-1998 INSERM U288, Faculté de Médecine Pitié-Salpêtrière, Paris,
•    1998- 2007 INSERM U513, Faculté de Médecine de Créteil, France
•    2007- Currently INSERM U952, CNRS UMR 7224, UPMC, Paris
•    2008- Currently, Douglas Research Center, Dept of Psychiatry, McGill University, Montreal, Canada


Caroline Fasano (research assistant)
Mathieu Favier (Postdoc)
Diana Sakae (Postdoc)
Maya Marcus-Sells (PhD)
Julia Pedersen (PhD, cosupervision Asa Mackenzie Upsala Sweden)
Érika Vigneault (Technician/lab manager)
Eve-Marie Charbonneau (Technician)


Recent Publications

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